Barnabas Fund is expanding its Exodus campaign to rescue another 3,400 Christians from Sudan.
The country has become increasingly hostile to Southern Sudanese, who are largely Christian, since South Sudan gained independence in 2011.
The expansion of the Exodus campaign has been made possible by the generosity of donations from supporters but Barnabas Fund said it still needs another £76,000 to cover the full costs of the rescue.
The organisation, which supports persecuted Christians, has airlifted and bussed nearly 5,000 vulnerable Christians to South Sudan.
It says Christians in the overwhelmingly Muslim Sudan are facing oppression and hostility under an increasingly aggressive Islamist government that wants to eradicate the Christian presence from the North.
Around two thirds of the Christians rescued so far have been widows and children, and they will remain a priority in the next evacuation.